The following study was conducted by Scientists from Department of Functional Neuroanatomy, Institute for Anatomy and Cell Biology, Heidelberg University, Im Neuenheimer Feld, Heidelberg, Germany; Institute of Physical and Theoretical Chemistry, Goethe-University Frankfurt, Max-von-Laue-Str, Frankfurt, Germany. Study is published in Nature Communications Journal as detailed below.
Nature Communications; Volume 11, Article Number: 1552; (2020)
Automated Highly Multiplexed Super-Resolution Imaging of Protein Nano-Architecture in Cells and Tissues
Abstract
Understanding the nano-architecture of protein machines in diverse subcellular compartments remains a challenge despite rapid progress in super-resolution microscopy. While single-molecule localization microscopy techniques allow the visualization and identification of cellular structures with near-molecular resolution, multiplex-labeling of tens of target proteins within the same sample has not yet been achieved routinely. However, single sample multiplexing is essential to detect patterns that threaten to get lost in multi-sample averaging. Here, we report maS3TORM (multiplexed automated serial staining stochastic optical reconstruction microscopy), a microscopy approach capable of fully automated 3D direct STORM (dSTORM) imaging and solution exchange employing a re-staining protocol to achieve highly multiplexed protein localization within individual biological samples. We demonstrate 3D super-resolution images of 15 targets in single cultured cells and 16 targets in individual neuronal tissue samples with <10 nm localization precision, allowing us to define distinct nano-architectural features of protein distribution within the presynaptic nerve terminal.
Source:
Nature Communications
URL: https://www.nature.com/articles/s41467-020-15362-1
Citation:
Klevanski, M., F. Herrmannsdoerfer, et al. (2020). “Automated highly multiplexed super-resolution imaging of protein nano-architecture in cells and tissues.” Nature Communications 11(1): 1552.
