The following study was conducted by Scientists from Department of Nanomedicine, Center for inflammation and Epigenetics, Houston Methodist Research Institute, Houston, USA; The Third Xiangya Hospital, Central South University, Changsha, China; Department of Cell and Developmental Biology, Weill Cornell Medical College, New York, USA; The Keck School of Medicine, University of Southern California, Los Angeles, USA. Study is published in Cell Reports Journal – Cell Press Publishing as detailed below.
Cell Reports Journal – Cell Press Publishing (2020)
Evaluation of Single-Cell Cytokine Secretion and Cell-Cell Interactions with a Hierarchical Loading Microwell Chip
Highlights
- A hierarchical loading microwell chip (HL-Chip) pairs objects with high efficiency
- A high-throughput assay enables single-cell cytokine detection and cell retrieval
- The assay system enables simultaneous detection of cytotoxicity and cytokine
- The high-throughput HL-Chip enables screening of functional T cells
Summary
Comprehensive evaluation of single T cell functions such as cytokine secretion and cytolysis of target cells is greatly needed in adoptive cell therapy (ACT) but has never been fully fulfilled by current approaches. Herein, we develop a hierarchical loading microwell chip (HL-Chip) that aligns multiple cells and functionalized beads in a high-throughput microwell array with single-cell/bead precision based on size differences. We demonstrate the potential of the HL-Chip in evaluating single T cell functions by three applications: high-throughput longitudinal secretory profiling of single T cells, large-scale evaluation of cytolytic activity of single T cells, and integrated T cell-tumor cell interactions. The HL-Chip is a simple and robust technology that constructs arrays of defined cell/object combinations for multiple measurements and material retrieval.
Source:
Cell Reports – Cell Press Publishing
URL: https://www.cell.com/cell-reports/fulltext/S2211-1247(20)30523-4
Citation:
Zhou, Y., N. Shao, et al. (2020). “Evaluation of Single-Cell Cytokine Secretion and Cell-Cell Interactions with a Hierarchical Loading Microwell Chip.” Cell Reports 31(4).
