The following study was conducted by Scientists from Department of Biomedical Engineering, Columbia University, New York, USA; Biotech Research & Innovation Centre, University of Copenhagen, Denmark; Department of Radiation Oncology, Stanford University, Palo Alto, USA; Columbia University Medical Center, New York, USA. Study is published in iScience Journal – Cell Press Publishing as detailed below.
iScience Journal – Cell Press Publishing (2020)
Injectable Therapeutic Organoids Using Sacrificial Hydrogels
Highlights
- Therapeutic, prevascularized organoids were formed in a sacrificial scaffold
- The organoids are highly reproducible and grown in a high-throughput manner
- The organoids rapidly formed perfusing vasculature in healthy mice
- Therapeutic potential was assessed in a mouse model of peripheral artery disease
Summary
Organoids are becoming widespread in drug-screening technologies but have been used sparingly for cell therapy as current approaches for producing self-organized cell clusters lack scalability or reproducibility in size and cellular organization. We introduce a method of using hydrogels as sacrificial scaffolds, which allow cells to form self-organized clusters followed by gentle release, resulting in highly reproducible multicellular structures on a large scale. We demonstrated this strategy for endothelial cells and mesenchymal stem cells to self-organize into blood-vessel units, which were injected into mice, and rapidly formed perfusing vasculature. Moreover, in a mouse model of peripheral artery disease, intramuscular injections of blood-vessel units resulted in rapid restoration of vascular perfusion within seven days. As cell therapy transforms into a new class of therapeutic modality, this simple method—by making use of the dynamic nature of hydrogels—could offer high yields of self-organized multicellular aggregates with reproducible sizes and cellular architectures.
Source:
iScience – Cell Press Publishing
URL: https://www.cell.com/iscience/fulltext/S2589-0042(20)30237-6
Citation:
Rossen, N. S., P. N. Anandakumaran, et al. (2020). “Injectable Therapeutic Organoids Using Sacrificial Hydrogels.” iScience 23(5).
