The following study was conducted by Scientists from Epigenetics Programme, Mass Spectrometry Facility, The Babraham Institute, Cambridge, UK; Bioinformatics Group, The Babraham Institute, Cambridge, UK; Wellcome-Medical Research Council Cambridge Stem Cell Institute, Cambridge, UK. Study is published in Stem Cell Reports Journal – Cell Press Publishing as detailed below.
Stem Cell Reports Journal – Cell Press Publishing (2020)
Cell-Surface Proteomics Identifies Differences in Signaling and Adhesion Protein Expression between Naive and Primed Human Pluripotent Stem Cells
Highlights
- Direct measurement of plasma membrane proteins in two human pluripotent states
- Proteomic resource contains quantitative analysis of signaling and adhesion proteins
- Multiple cytokine receptors upstream of JAK-STAT pathway identified in naive cells
- Functional experiments reveal FOLR1 and SUSD2 are not required for naive pluripotency
Summary
Naive and primed human pluripotent stem cells (hPSC) provide valuable models to study cellular and molecular developmental processes. The lack of detailed information about cell-surface protein expression in these two pluripotent cell types prevents an understanding of how the cells communicate and interact with their microenvironments. Here, we used plasma membrane profiling to directly measure cell-surface protein expression in naive and primed hPSC. This unbiased approach quantified over 1,700 plasma membrane proteins, including those involved in cell adhesion, signaling, and cell interactions. Notably, multiple cytokine receptors upstream of JAK-STAT signaling were more abundant in naive hPSC. In addition, functional experiments showed that FOLR1 and SUSD2 proteins are highly expressed at the cell surface in naive hPSC but are not required to establish human naive pluripotency. This study provides a comprehensive stem cell proteomic resource that uncovers differences in signaling pathway activity and has identified new markers to define human pluripotent states.
Source:
Stem Cell Reports – Cell Press Publishing
URL: https://www.cell.com/stem-cell-reports/fulltext/S2213-6711(20)30107-7
Citation:
Wojdyla, K., A. J. Collier, et al. (2020). “Cell-Surface Proteomics Identifies Differences in Signaling and Adhesion Protein Expression between Naive and Primed Human Pluripotent Stem Cells.” Stem Cell Reports 14(5): 972-988.
