The following study was conducted by Scientists from Department of Pathology, University of Pittsburgh, Pittsburgh, USA; Department of Surgery and Science, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan; Department of Hepatobiliary Surgery, Peking University People’s Hospital, Beijing, China; Department of Physiology and Biophysics, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil; Department of Hepatobiliary Surgery, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, China; Department of Bioengineering and Department of Developmental Biology, University of Pittsburgh, Pittsburgh, USA; Life Cell Corporation, Branchburg, NJ, USA; Center for Biologic Imaging, University of Pittsburgh Medical School, Pittsburgh, USA; McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, USA; Pittsburgh Liver Research Center, University of Pittsburgh, Pittsburgh, USA; Department of Radiology, Michigan State University, East Lansing, USA; Facultad de Ciencias de la Salud, Carrera de Medicina, Universidad Maimo´ nides, Ciudad Auto´ noma de Buenos Aires and Consejo Nacional de Investigaciones Cientı´ficas y Te´ cnicas (CONICET), Ciudad Auto´ noma de Buenos Aires, Buenos Aires, Argentina; Department of Surgery, School of Medicine, Keio University, Tokyo, Japan; Division of Animal Genetics, Laboratory Animal Research Center, Institute of Medical Science, University of Tokyo, Tokyo, Japan; Department of Surgery, Children’s Hospital of Pittsburgh of UPMC, University of Pittsburgh, Pittsburgh, USA. Study is published in Cell Reports Journal – Cell Press Publishing as detailed below.
Cell Reports Journal – Cell Press Publishing (2020)
Assembly and Function of a Bioengineered Human Liver for Transplantation Generated Solely from Induced Pluripotent Stem Cells
Highlights
- Organ-like microenvironment further matures human iPSC mini livers
- Human vascular and biliary network can be engineered in decellularized liver scaffolds
- Human iPSC-mini-liver microstructure has similarities to human liver
- Human iPSC-derived mini livers can be transplanted in immunodeficient rats
Summary
The availability of an autologous transplantable auxiliary liver would dramatically affect the treatment of liver disease. Assembly and function in vivo of a bioengineered human liver derived from induced pluripotent stem cells (iPSCs) has not been previously described. By improving methods for liver decellularization, recellularization, and differentiation of different liver cellular lineages of human iPSCs in an organ-like environment, we generated functional engineered human mini livers and performed transplantation in a rat model. Whereas previous studies recellularized liver scaffolds largely with rodent hepatocytes, we repopulated not only the parenchyma with human iPSC-hepatocytes but also the vascular system with human iPS-endothelial cells, and the bile duct network with human iPSC-biliary epithelial cells. The regenerated human iPSC-derived mini liver containing multiple cell types was tested in vivo and remained functional for 4 days after auxiliary liver transplantation in immunocompromised, engineered (IL2rg−/−) rats.
Source:
Cell Reports Journal – Cell Press Publishing
URL: https://www.cell.com/cell-reports/fulltext/S2211-1247(20)30688-4
Citation:
Takeishi, K., A. Collin de l’Hortet, et al. (2020). “Assembly and Function of a Bioengineered Human Liver for Transplantation Generated Solely from Induced Pluripotent Stem Cells.” Cell Reports 31(9).
