The following study was conducted by Scientists from Department of Biomedical Informatics, Stanford University, Palo Alto; Department of Biomedical Engineering, University of Michigan, Ann Arbor; Biointerfaces Institute, University of Michigan, Ann Arbor; Department of Molecular & Integrative Physiology, University of Michigan, Ann Arbor; Program in Cancer Biology, University of Michigan, Ann Arbor; Program in Cellular and Molecular Biology, University of Michigan, Ann Arbor; Parker H. Petit Institute of Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta; School of Biological Sciences, Georgia Institute of Technology, Atlanta, GA; Wallace Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, GA; Department of Biomedical Engineering, University of Virginia, Charlottesville, VA, USA. Study is published in Cell Reports Journal – Cell Press Publishing as detailed below.
Cell Reports Journal – Cell Press Publishing (2020)
Dissecting Murine Muscle Stem Cell Aging through Regeneration Using Integrative Genomic Analysis
Highlights
- Chromatin enzymes that mediate heterochromatin packaging vary in aging
- Distinct activity of one-carbon and retinol pathways observed in aged satellite cells
- Changes in transcription factor binding in aged satellite cells post-injury
Summary
During aging, there is a progressive loss of volume and function in skeletal muscle that impacts mobility and quality of life. The repair of skeletal muscle is regulated by tissue-resident stem cells called satellite cells (or muscle stem cells [MuSCs]), but in aging, MuSCs decrease in numbers and regenerative capacity. The transcriptional networks and epigenetic changes that confer diminished regenerative function in MuSCs as a result of natural aging are only partially understood. Herein, we use an integrative genomics approach to profile MuSCs from young and aged animals before and after injury. Integration of these datasets reveals aging impacts multiple regulatory changes through significant differences in gene expression, metabolic flux, chromatin accessibility, and patterns of transcription factor (TF) binding activities. Collectively, these datasets facilitate a deeper understanding of the regulation tissue-resident stem cells use during aging and healing.
Source:
Cell Reports Journal – Cell Press Publishing
URL: https://www.cell.com/cell-reports/fulltext/S2211-1247(20)30945-1
Citation:
Shcherbina, A., J. Larouche, et al. (2020). “Dissecting Murine Muscle Stem Cell Aging through Regeneration Using Integrative Genomic Analysis.” Cell Reports 32(4).
