The following study was conducted by Scientists from Boston University and Boston Medical Center; Boston University School of Medicine; Cystic Fibrosis Foundation Therapeutics Lab, Lexington; Boston University School of Medicine, USA. Study is published in Nature Communications Journal as detailed below.
Nature Communications (2020); Volume:11, Article number: 215
Generation of Mesenchyme Free Intestinal Organoids from Human Induced Pluripotent Stem Cells
Abstract
Efficient generation of human induced pluripotent stem cell (hiPSC)-derived human intestinal organoids (HIOs) would facilitate the development of in vitro models for a variety of diseases that affect the gastrointestinal tract, such as inflammatory bowel disease or Cystic Fibrosis. Here, we report a directed differentiation protocol for the generation of mesenchyme-free HIOs that can be primed towards more colonic or proximal intestinal lineages in serum-free defined conditions. Using a CDX2eGFP iPSC knock-in reporter line to track the emergence of hindgut progenitors, we follow the kinetics of CDX2 expression throughout directed differentiation, enabling the purification of intestinal progenitors and robust generation of mesenchyme-free organoids expressing characteristic markers of small intestinal or colonic epithelium. We employ HIOs generated in this way to measure CFTR function using cystic fibrosis patient-derived iPSC lines before and after correction of the CFTR mutation, demonstrating their future potential for disease modeling and therapeutic screening applications.
Source:
Nature Communications
URL: https://www.nature.com/articles/s41467-019-13916-6
Citation:
Mithal, A., Capilla, A., Heinze, D. et al. Generation of mesenchyme free intestinal organoids from human induced pluripotent stem cells. Nat Commun 11, 215 (2020). https://doi.org/10.1038/s41467-019-13916-6.
