The following study was conducted by Scientists from CAS Key Laboratory of Nanosystem and Hierarchical Fabrication, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, Beijing, China; University of Chinese Academy of Sciences, Beijing, China; Sino-Danish College, Sino-Danish Center for Education and Research, University of Chinese Academy of Sciences, Beijing, China; Beijing Jishuitan Hospital, Department of Laboratory Medicine, Peking University Fourth School of Clinical Medicine, Beijing, China; National & Local Joint Engineering Research Center of Biodiagnosis and Biotherapy, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an, China; School of Materials Science and Engineering, Zhengzhou University, Zhengzhou, China. Study is published in Nature Communications Journal as detailed below.
Nature Communications; Volume 12, Article Number: 358 (2021)
A DNA Origami-Based Aptamer Nanoarray for Potent and Reversible Anticoagulation in Hemodialysis
Abstract
Effective and safe hemodialysis is essential for patients with acute kidney injury and chronic renal failures. However, the development of effective anticoagulant agents with safe antidotes for use during hemodialysis has proven challenging. Here, we describe DNA origami-based assemblies that enable the inhibition of thrombin activity and thrombus formation. Two different thrombin-binding aptamers decorated DNA origami initiates protein recognition and inhibition, exhibiting enhanced anticoagulation in human plasma, fresh whole blood and a murine model. In a dialyzer-containing extracorporeal circuit that mimicked clinical hemodialysis, the origami-based aptamer nanoarray effectively prevented thrombosis formation. Oligonucleotides containing sequences complementary to the thrombin-binding aptamers can efficiently neutralize the anticoagulant effects. The nanoarray is safe and immunologically inert in healthy mice, eliciting no detectable changes in liver and kidney functions or serum cytokine concentration. This DNA origami-based nanoagent represents a promising anticoagulant platform for the hemodialysis treatment of renal diseases.
Source:
Nature Communications
URL: https://www.nature.com/articles/s41467-020-20638-7
Citation:
Zhao, S., Tian, R., Wu, J. et al. A DNA origami-based aptamer nanoarray for potent and reversible anticoagulation in hemodialysis. Nat Commun 12, 358 (2021). https://doi.org/10.1038/s41467-020-20638-7
