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Efficient profiling of the plasma proteome with multi-nanoparticle protein corona

By 9th November 2020No Comments

The following study was conducted by Scientists from Seer, Inc., Redwood City, CA, USA; Broad Institute of MIT and Harvard, Cambridge, MA, USA; David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, USA; Sloan School and Operations Research Center, Massachusetts Institute of Technology, Cambridge, MA, USA; Center for Nanomedicine and Department of Anesthesiology, Brigham and Women’s Hospital, Harvard Medical School, Boston, MA, USA. Study is published in Nature Communications Journal as detailed below.

Nature Communications; Volume 11, Article Number: 3662; (2020)

Rapid, Deep and Precise Profiling of the Plasma Proteome with Multi-Nanoparticle Protein Corona


Large-scale, unbiased proteomics studies are constrained by the complexity of the plasma proteome. Here we report a highly parallel protein quantitation platform integrating nanoparticle (NP) protein coronas with liquid chromatography-mass spectrometry for efficient proteomic profiling. A protein corona is a protein layer adsorbed onto NPs upon contact with biofluids. Varying the physicochemical properties of engineered NPs translates to distinct protein corona patterns enabling differential and reproducible interrogation of biological samples, including deep sampling of the plasma proteome. Spike experiments confirm a linear signal response. The median coefficient of variation was 22%. We screened 43 NPs and selected a panel of 5, which detect more than 2,000 proteins from 141 plasma samples using a 96-well automated workflow in a pilot non-small cell lung cancer classification study. Our streamlined workflow combines depth of coverage and throughput with precise quantification based on unique interactions between proteins and NPs engineered for deep and scalable quantitative proteomic studies.


Nature Communications



Blume, J. E., W. C. Manning, et al. (2020). “Rapid, deep and precise profiling of the plasma proteome with multi-nanoparticle protein corona.” Nature Communications 11(1): 3662.