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Ultrasensitive Molecular Imaging by Target-Responsive Vasoactive Probes

By 11th September 2020No Comments

The following study was conducted by Scientists from Department of Biological Engineering, Massachusetts Institute of Technology, Massachusetts Ave, Cambridge, MA, USA; Harvard-MIT Health Sciences & Technology, Massachusetts Institute of Technology, Cambridge, USA; Department of Brain & Cognitive Sciences, Massachusetts Institute of Technology, Massachusetts Ave, Cambridge, USA; Department of Nuclear Science & Engineering, Massachusetts Institute of Technology, Massachusetts Ave, Cambridge, USA. Study is published in Nature Communications Journal as detailed below

Nature Communications; Volume 11, Article number: 2399 (2020)

Target-Responsive Vasoactive Probes for Ultrasensitive Molecular Imaging


The ability to monitor molecules volumetrically throughout the body could provide valuable biomarkers for studies of healthy function and disease, but noninvasive detection of molecular targets in living subjects often suffers from poor sensitivity or selectivity. Here we describe a family of potent imaging probes that can be activated by molecules of interest in deep tissue, providing a basis for mapping nanomolar-scale analytes without the radiation or heavy metal content associated with traditional molecular imaging agents. The probes are reversibly caged vasodilators that induce responses detectable by hemodynamic imaging; they are constructed by combining vasoactive peptides with synthetic chemical appendages and protein blocking domains. We use this architecture to create ultrasensitive biotin-responsive imaging agents, which we apply for wide-field mapping of targets in rat brains using functional magnetic resonance imaging. We also adapt the sensor design for detecting the neurotransmitter dopamine, illustrating versatility of this approach for addressing biologically important molecules.


Nature Communications



Ohlendorf, R., A. Wiśniowska, et al. (2020). “Target-responsive vasoactive probes for ultrasensitive molecular imaging.” Nature Communications 11(1): 2399.